Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage
- PMID: 17629788
- DOI: 10.1007/s11033-007-9103-5
Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage
Abstract
The techniques of homology cloning and anchored PCR were used to clone the elongation factor 2 (EF-2) gene from black tiger shrimp (Penaeus monodon). The full length cDNA of black tiger shrimp EF-2 (btsEF-2) contained a 5' untranslated region (UTR) of 73 bp, an ORF of 2541 bp encoding a polypeptide of 846 amino acids with an estimated molecular mass of 95 kDa, and a 3( UTR of 112 bp. The searches for protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of btsEF-2 was homological to the EF-2 of other species and even the mammalians. The conserved signature sequence of EF-2 gene family, GTPase effector domain and ADP-ribosylation domain were found in the btsEF-2 deduced amino acid sequence. The temporal expressions of gene in the different ovarian stages were measured by real time PCR. The mRNA expressions of the gene were constitutively expressed in ovary and different during the maturation stages. The result indicated that EF-2 gene was constitutively expressed and could play a critical role in the ovarian maturation stage.
Similar articles
-
Molecular cloning and characterization of a cyclin B gene on the ovarian maturation stage of black tiger shrimp (Penaeus monodon).Mol Biol Rep. 2023 Oct;50(Suppl 1):S1-S8. doi: 10.1007/s11033-006-9052-4. Epub 2007 Jan 24. Mol Biol Rep. 2023. PMID: 17245552
-
Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon).Mol Biol Rep. 2009 Jan;36(1):127-34. doi: 10.1007/s11033-007-9160-9. Epub 2007 Oct 13. Mol Biol Rep. 2009. PMID: 17934796
-
Molecular cloning and mRNA expression of cyclophilin A gene in black tiger shrimp (Penaeus monodon).Fish Shellfish Immunol. 2009 Jan;26(1):115-21. doi: 10.1016/j.fsi.2008.03.022. Epub 2008 Apr 7. Fish Shellfish Immunol. 2009. PMID: 19041262
-
Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon).Genet Mol Res. 2016 Feb 5;15(1). doi: 10.4238/gmr.15017367. Genet Mol Res. 2016. PMID: 26909956
-
Molecular analysis of the QM gene from Penaeus monodon and its expression on the different ovarian stages of development.Mol Biol Rep. 2011 Mar;38(3):1921-7. doi: 10.1007/s11033-010-0312-y. Epub 2010 Sep 25. Mol Biol Rep. 2011. PMID: 20872074
Cited by
-
Transcriptome analysis of red swamp crawfish Procambarus clarkii reveals genes involved in gonadal development.PLoS One. 2014 Aug 13;9(8):e105122. doi: 10.1371/journal.pone.0105122. eCollection 2014. PLoS One. 2014. PMID: 25118947 Free PMC article.
-
Molecular analysis of a ras-like nuclear (Ran) gene from Penaeus monodon and its expression at the different ovarian stages of development.Mol Biol Rep. 2012 Apr;39(4):3821-7. doi: 10.1007/s11033-011-1160-0. Epub 2011 Jul 12. Mol Biol Rep. 2012. PMID: 21748319
-
High salinity induced expression profiling of differentially expressed genes in shrimp (Penaeus monodon).Mol Biol Rep. 2014 Sep;41(9):6275-89. doi: 10.1007/s11033-014-3510-1. Epub 2014 Jun 29. Mol Biol Rep. 2014. PMID: 24973887
-
Molecular characterisation of colour formation in the prawn Fenneropenaeus merguiensis.PLoS One. 2013;8(2):e56920. doi: 10.1371/journal.pone.0056920. Epub 2013 Feb 18. PLoS One. 2013. PMID: 23441225 Free PMC article.
-
Molecular cloning and its expression of trachealess gene (As-trh) during development in brine shrimp, Artemia sinica.Mol Biol Rep. 2012 Feb;39(2):1659-65. doi: 10.1007/s11033-011-0905-0. Epub 2011 May 31. Mol Biol Rep. 2012. PMID: 21625858
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials