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. 2007 Sep;73(17):5692-7.
doi: 10.1128/AEM.00419-07. Epub 2007 Jul 13.

Pathotyping Escherichia coli by using miniaturized DNA microarrays

Muna F Anjum  1 , Muriel Mafura, Peter Slickers, Karin Ballmer, Peter Kuhnert, Martin J Woodward, Ralf Ehricht
Affiliations

Pathotyping Escherichia coli by using miniaturized DNA microarrays

Muna F Anjum et al. Appl Environ Microbiol. 2007 Sep.

Abstract

The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.

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Figures

FIG. 1.
FIG. 1.
Optimization of the genomic concentration used in this study. The optimal concentration of genomic DNA from EDL933 used for the detection of genes on the virulence oligonucleotide miniaturized microarray chip was assessed using (a) 2 μg, (b) 1 μg, (c) 0.5 μg, (d) 0.1 μg, and (e) 0.05 μg of DNA. The six biotinylated marker spots (C) are visible in all arrays. A concentration of 0.135 μM per primer in the stock solution and 200 pg/μl of poly-horseradish peroxidase-streptavidin conjugate were used for these assays.
See this image and copyright information in PMC

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