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. 1991 Dec 15;88(24):11388-92.
doi: 10.1073/pnas.88.24.11388.

Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis

J C Cook  1 , P B Chock
Affiliations

Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis

J C Cook et al. Proc Natl Acad Sci U S A. .

Abstract

Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.

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