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. 2007 Sep;189(18):6723-6.
doi: 10.1128/JB.00279-07. Epub 2007 Jul 13.

Characterization of in vitro chlamydial cultures in low-oxygen atmospheres

Nicolai Juul  1 , Helene Jensen, Malene Hvid, Gunna Christiansen, Svend Birkelund
Affiliations

Characterization of in vitro chlamydial cultures in low-oxygen atmospheres

Nicolai Juul et al. J Bacteriol. 2007 Sep.

Abstract

To mimic in vivo conditions during chlamydial infections, Chlamydia trachomatis serovar D and Chlamydia pneumoniae CWL029 were cultured in low-oxygen atmospheres containing 4% O(2), with parallel controls cultured in atmospheric air. Both were enriched with 5% CO(2). The results showed a dramatic increase in the growth of C. pneumoniae but not of C. trachomatis.

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Figures

FIG. 1.
FIG. 1.
Inclusion size at 4% and 20% oxygen. (A and B) Increase in inclusion size of C. trachomatis (A) and C. pneumoniae (B) when cultured at 4% and 20% oxygen. (C to F) Representative confocal images of C. trachomatis and C. pneumoniae inclusions. Shown are C. trachomatis at 4% (C) and 20% (E) oxygen and C. pneumoniae at 4% (D) and 20% (F) oxygen. Bars represent 10 μm.
FIG. 2.
FIG. 2.
Growth curves for C. trachomatis up to 48 hpi and C. pneumoniae up to 84 hpi when cultured at 4% and 20% oxygen. (A and C) Temporal increase in C. trachomatis IFU (A) and GC (C). (B and D) Temporal increase in C. pneumoniae IFU (B) and GC (D).
See this image and copyright information in PMC

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