Review
doi: 10.1007/978-1-59745-388-2_2.
Continuous-exchange protein-synthesizing systems
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- PMID: 17634595
- DOI: 10.1007/978-1-59745-388-2_2
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Review
Continuous-exchange protein-synthesizing systems
Methods Mol Biol.
2007.
Abstract
Protein synthesis in cell-free systems is an emerging technology already competing with in vivo expression methods. In this chapter the basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat germ translation and transcription-translation systems are described. The ways to improve substrate supply in cell-free systems and mRNA design for eukaryotic system are discussed. Correct folding of the synthesized protein is demonstrated and discussed in detail.
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