Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network
- PMID: 17652478
- PMCID: PMC2045238
- DOI: 10.1128/JCM.00697-07
Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network
Abstract
Influenza surveillance networks must detect early the viruses that will cause the forthcoming annual epidemics and isolate the strains for further characterization. We obtained the highest sensitivity (95.4%) with a diagnostic tool that combined a shell-vial assay and reverse transcription-PCR on cell culture supernatants at 48 h, and indeed, recovered the strain.
References
-
- Choo, Y. J., and S. J. Kim. 2006. Detection of human adenoviruses and enteroviruses in Korean oysters using cell culture, integrated cell culture-PCR, and direct PCR. J. Microbiol. 44:162-170. - PubMed
-
- Dunn, J. J., C. Gordon, C. Kelley, and K. C. Carroll. 2003. Comparison of the Denka-Seiken INFLU A.B-Quick and BD Directigen Flu A+B kits with direct fluorescent-antibody staining and shell vial culture methods for rapid detection of influenza viruses. J. Clin. Microbiol. 41:2180-2183. - PMC - PubMed
-
- Ellis, J. S., and M. C. Zambon. 2002. Molecular diagnosis of influenza. Rev. Med. Virol. 12:375-389. - PubMed
