Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Sep;45(9):3109-10.
doi: 10.1128/JCM.00697-07. Epub 2007 Jul 25.

Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network

Affiliations

Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network

Mercedes Pérez-Ruiz et al. J Clin Microbiol. 2007 Sep.

Abstract

Influenza surveillance networks must detect early the viruses that will cause the forthcoming annual epidemics and isolate the strains for further characterization. We obtained the highest sensitivity (95.4%) with a diagnostic tool that combined a shell-vial assay and reverse transcription-PCR on cell culture supernatants at 48 h, and indeed, recovered the strain.

PubMed Disclaimer

References

    1. Boivin, G., I. Hardy, and A. Kress. 2001. Evaluation of a rapid optical immunoassay for influenza viruses (FLU OIA test) in comparison with cell culture and reverse transcription-PCR. J. Clin. Microbiol. 39:730-732. - PMC - PubMed
    1. Choo, Y. J., and S. J. Kim. 2006. Detection of human adenoviruses and enteroviruses in Korean oysters using cell culture, integrated cell culture-PCR, and direct PCR. J. Microbiol. 44:162-170. - PubMed
    1. Dunn, J. J., C. Gordon, C. Kelley, and K. C. Carroll. 2003. Comparison of the Denka-Seiken INFLU A.B-Quick and BD Directigen Flu A+B kits with direct fluorescent-antibody staining and shell vial culture methods for rapid detection of influenza viruses. J. Clin. Microbiol. 41:2180-2183. - PMC - PubMed
    1. Ebihara, T., R. Endo, X. Ma, N. Ishiguro, and H. Kikuta. 2005. Detection of human metapneumovirus antigens in nasopharyngeal secretions by an immunofluorescent-antibody test. J. Clin. Microbiol. 43:1138-1141. - PMC - PubMed
    1. Ellis, J. S., and M. C. Zambon. 2002. Molecular diagnosis of influenza. Rev. Med. Virol. 12:375-389. - PubMed

Publication types

MeSH terms