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. 2007 Jul 3;40(3):77-81.
doi: 10.1267/ahc.06026.

Immunodetection of thyroid hormone receptor (alpha1/alpha2) in the rat uterus and oviduct

Jale Oner  1 , Hakan Oner
Affiliations

Immunodetection of thyroid hormone receptor (alpha1/alpha2) in the rat uterus and oviduct

Jale Oner et al. Acta Histochem Cytochem. .

Abstract

The aim of this study was to investigate the immunolocalization and the existence of thyroid hormone receptors (THR) (alpha1/alpha2) in rat uterus and oviduct. For this purpose 6 female Wistar albino rats found in estrous period were used. Tissue samples fixed in 10% neutral formalin were examined immunohistochemically. Sections were incubated with primary mouse-monoclonal THR (alpha1/alpha2) antibody. In uterus, THR (alpha1/alpha2) immunoreacted strongly with uterine luminal epithelium, endometrial gland epithelium and endometrial stromal cells and, moderately with myometrial smooth muscle. In oviduct, they were observed moderately in the epithelium of the tube and the smooth muscle cells of the muscular layer. In conclusion, the presence of THR in uterus and oviduct suggests that these organs are an active site of thyroid hormones.

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Figures

Fig 1
Fig 1
Thyroid hormone receptor (THR) (alpha1/alpha2) immunolocalization in rat uterus (A–D) and rat oviduct (F). E, endometrium; M, myometrium; UL, uterine lumen; yellow arrows, capillaries; green arrows, endometrial glandular epithelium; black arrows, uterine luminal epithelium; s, endometrial stroma; LE, lamina epithelialis of oviduct; LP, lamina propria of oviduct; TM, tunica muscularis of oviduct. Negative controls for THR (alpha1/alpha2) staining are shown as an insert. Magnifications: ×10 (A); ×100 (B–E); ×40 (F).
See this image and copyright information in PMC

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