doi: 10.1007/978-1-59745-277-9_16.
A flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus-transformed cells
- PMID: 17656786
- DOI: 10.1007/978-1-59745-277-9_16
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A flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus-transformed cells
Methods Mol Med.
2007.
Abstract
Natural-killer (NK) cells play an important role in recognizing and eliminating virally infected and transformed cells. To study this process, convenient assays for NK-cell function are required. Conventional NK-cell activity assays measure the release of 51Cr from prelabeled target cells following membrane disruption. This chapter describes nonradiometric assays for NK-cell killing of adenovirus-transformed human cells that can be applied to multiple cell samples using flow cytometry.
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