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. 2008 Feb;128(2):485-8.
doi: 10.1038/sj.jid.5700991. Epub 2007 Aug 2.

The importance of long-term monitoring to evaluate the microvascular response to light-based therapies

Bernard Choi, Wangcun Jia, Jennifer Channual, Kristen M Kelly, Justin Lotfi

The importance of long-term monitoring to evaluate the microvascular response to light-based therapies

Bernard Choi et al. J Invest Dermatol. 2008 Feb.
No abstract available

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Conflict of interest statement

CONFLICT OF INTEREST

The authors state no conflict of interest.

Figures

Figure 1
Figure 1. Microvascular blood flow response after pulsed laser irradiation
(a) Time sequence of wide-field color reflectance images (top row) and corresponding speckle flow index (SFI) images (bottom row) acquired over a 21-day monitoring period after pulsed laser irradiation of selected sites. Two arteriole-venule pairs (dashed circles in “Before” image) were irradiated with simultaneous 532 and 1064nm laser pulses (upper circle—five 1-ms laser pulses at 27 Hz repetition rate, 2 J/cm2 at 532 nm, 3.6 J/cm2 at 1064 nm; lower circle—single 1-ms laser pulse, 4 J/cm2 at 532 nm, 7.2 J/cm2 at 1064 nm). Vascular remodeling and blood flow dynamics were evident during the 21-day monitoring period, with the day 0 and day 21 structural images having similar appearances. (b) Quantitative evaluation of selected blood vessel regions of interest. In all three regions, the blood flow was shut down immediately, followed by eventual reperfusion to near-baseline blood flow levels. Color reflectance image dimensions (H×V): 13×10mm2; SFI image dimensions: 9×7mm2.
Figure 2
Figure 2. Microvascular blood flow response, assessed with laser speckle imaging, after photodynamic therapy
An ~4-mm diameter air bubble was present in the day 0 images, but it had no apparent effect on the speckle flow index (SFI) values. Benzoporphyrin monoacid ring A (1.5 mg/kg body mass) was administered via a jugular vein catheter. Fifteen minutes after benzoporphyrin monoacid ring A injection, continuous wave laser irradiation of the entire window chamber was performed with an argon-pumped dye laser (576 nm, 100mW/cm2 irradiance, 96 J/cm2 radiant exposure). Irradiation was performed on the epidermal side of the chamber. A hyperemic response was observed immediately after photodynamic therapy, consistent with previous data (Smith et al., 2006). At day 1, a large reduction in blood flow was observed, followed by a progressive increase in blood flow in the larger blood vessels. Image dimensions (H×V): 9×7mm2.
See this image and copyright information in PMC

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