Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric detection

Abstract

We have developed an improved analytical method for the determination of sugar phosphates using sodium carbonate (Na(2)CO(3)) for high-performance anion-exchange chromatography-pulsed amperometric detection. The target analytes were separated completely within 10 min using eluent containing 20 mM NaOH and 35 mM Na(2)CO(3). The limit of detection (S/N=3) and quantitation (S/N=10) for analytes were 10-30 ng/mL and 35-100 ng/mL, respectively. Linear dynamic range was 1-30 microg/mL (r(2)> or =0.9998). The RSDs for intra- and inter-day assays were found to be of satisfactory results (0.23-3.09%), and the recoveries from blood spots were 97.62-99.69%.