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. 2007 Jul;58(1):65-69.
doi: 10.1002/mrm.21253.

In vivo 13 carbon metabolic imaging at 3T with hyperpolarized 13C-1-pyruvate

S J Kohler  1 , Y Yen  1 , J Wolber  2 , A P Chen  3 , M J Albers  4 , R Bok  3 , V Zhang  3 , J Tropp  1 , S Nelson  3   4 , D B Vigneron  3   4 , J Kurhanewicz  3   4 , R E Hurd  1
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Free article

In vivo 13 carbon metabolic imaging at 3T with hyperpolarized 13C-1-pyruvate

S J Kohler et al. Magn Reson Med. 2007 Jul.
Free article

Abstract

We present for the first time dynamic spectra and spectroscopic images acquired in normal rats at 3T following the injection of (13)C-1-pyruvate that was hyperpolarized by the dynamic nuclear polarization (DNP) method. Spectroscopic sampling was optimized for signal-to-noise ratio (SNR) and for spectral resolution of (13)C-1-pyruvate and its metabolic products (13)C-1-alanine, (13)C-1-lactate, and (13)C-bicarbonate. Dynamic spectra in rats were collected with a temporal resolution of 3 s from a 90-mm axial slab using a dual (1)H-(13)C quadrature birdcage coil to observe the combined effects of metabolism, flow, and T(1) relaxation. In separate experiments, spectroscopic imaging data were obtained during a 17-s acquisition of a 20-mm axial slice centered on the rat kidney region to provide information on the spatial distribution of the metabolites. Conversion of pyruvate to lactate, alanine, and bicarbonate occurred within a minute of injection. Alanine was observed primarily in skeletal muscle and liver, while pyruvate, lactate, and bicarbonate concentrations were relatively high in the vasculature and kidneys. In contrast to earlier work at 1.5 T, bicarbonate was routinely observed in skeletal muscle as well as the kidney and vasculature.

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References

REFERENCES

    1. Abragam A, Goldman M. Principles of dynamic nuclear polarization. Rep Prog Phys 1978; 41: 395-467.
    1. Goldman M. Spin temperature and nuclear magnetic resonance in solids. Oxford: Oxford University Press; 1970.
    1. deBoer W, Borghini M, Morimoto K, Niinikoski TO, Udo T, Low J. Dynamic polarization of protons, deuterons, and carbon-13 nuclei: thermal contact between nuclear spins and an electron spin-spin interaction reservoir. Low Temp Phys 1974; 15: 249-267.
    1. deBoer W, Niinikoski TO. Dynamic proton polarization in propanediol below 0.5 K. Nucl Instrum Methods 1974; 114: 495-498.
    1. Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG. Polarization-enhanced NMR spectroscopy of biomolecules frozen in solution. Science 1997; 276: 930-932.

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