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. 2007 Jul 14;13(26):3625-30.
doi: 10.3748/wjg.v13.i26.3625.

Relationship of hepatitis B virus infection of placental barrier and hepatitis B virus intra-uterine transmission mechanism

Han Bai  1 , Lin Zhang, Li Ma, Xiao-Guang Dou, Guo-He Feng, Gui-Zhen Zhao
Affiliations

Relationship of hepatitis B virus infection of placental barrier and hepatitis B virus intra-uterine transmission mechanism

Han Bai et al. World J Gastroenterol. .

Abstract

Aim: To explore the mechanism of intra-uterine transmission, the HBV infection status of placental tissue and in vitro cultured placental trophoblastic cells was tested through in vivo and in vitro experiments.

Methods: A variety of methods, such as ELISA, RT-PCR, IHC staining and immunofluorescent staining were employed to test the HBV marker positive pregnant women's placenta and in vitro cultured placental trophoblastic cells.

Results: The HBV DNA levels in pregnant women's serum and fetal cord blood were correlated. For those cord blood samples positive for HBV DNA, their maternal blood levels of HBV DNA were at a high level. The HBsAg IHC staining positive cells could be seen in the placental tissues and the presence of HBV DNA detected. After co-incubating the trophoblastic cells and HBV DNA positive serum in vitro, the expressions of both HBsAg and HBV DNA could be detected.

Conclusion: The mechanism of HBV intra-uterine infection may be due to that HBV breaches the placental barrier and infects the fetus.

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Figures

Figure 1
Figure 1
The IHC staining results of the placenta of HBV carrier mothers. A: HBsAg positive cells in the vascular endothelial cell of the placenta villi (× 40); B: HBsAg positive cells in the trophoblastic cell of on the surface of placenta villi (× 40).
Figure 2
Figure 2
Immunofluorescence staining results of trophoblastic cells of different co-incubation time with HBV positive serum. A: 8 h; B: 24 h; C: 48 h.
Figure 3
Figure 3
HBV DNA detection results in the trophoblastic cell by RT-PCR. A: standard curve; B: detective result.
See this image and copyright information in PMC

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