Study of therapeutic potential of the experimental pseudomonas bacteriophage preparation

Abstract

Wide expansion of the infections caused by multi-antibiotic resistant strains of P. aeruginosa revived the idea of phage therapy with pseudomonas phage preparations for treatment and prevention of the bacterial infection diseases. The purpose of this study was examination of an experimental series of the therapeutic-prophylactic pseudomonas bacteriophage preparation, with wide spectra of lytical activity and high therapeutic potential. Newly isolated phage clones of P. aeruginosa were studied by the basic tests (such as host range, lysis stability, physiological and immunogenic properties of the phages, host dependent restriction/modification phenomena and automatic reproduction ability of the phages on the UV inactivated strains) determining their virulent nature. An experimental series of the therapeutic-prophylactic pseudomonas phage preparation were developed from the genuine virulent phage clones CF1/1; CF1/7; P.a.N1, P.a.N2 and P.a.N4. The phage preparation successfully passed in vitro (efficacy, sterility, stability) and in vivo (safety, definition of therapeutic potential) controls on experimental animals (white mice). The host range of the experimental pseudomonas phage preparation equals 99.5% of 206 strains of P. aeruginosa. Preclinical testing of the experimental pseudomonas phage preparation on white mice revealed that the therapeutic efficacy of the phage preparation was higher (80-100%) than that of the antibiotic-ciprinol (50-80%). Noteworthy, 100% therapeutic efficacy was observed after combined application of the antibiotic and the phage preparation.