doi: 10.1016/j.vaccine.2007.06.036.
Epub 2007 Jul 10.
Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation
Affiliations
- PMID: 17669562
- DOI: 10.1016/j.vaccine.2007.06.036
Item in Clipboard
Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation
Vaccine.
.
Abstract
We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 microg of DNA via EP was equivalent to 50 microg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-gamma responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.
Similar articles
-
Plasmodium: mammalian codon optimization of malaria plasmid DNA vaccines enhances antibody responses but not T cell responses nor protective immunity.Exp Parasitol. 2009 Jun;122(2):112-23. doi: 10.1016/j.exppara.2009.02.010. Epub 2009 Feb 26. Exp Parasitol. 2009. PMID: 19249301
-
Targeting antigen to MHC Class I and Class II antigen presentation pathways for malaria DNA vaccines.Immunol Lett. 2007 Aug 15;111(2):92-102. doi: 10.1016/j.imlet.2007.05.007. Epub 2007 Jun 18. Immunol Lett. 2007. PMID: 17604849
-
DNA immunization by Plasmodium falciparum liver-stage antigen 3 induces protection against Plasmodium yoelii sporozoite challenge.Infect Immun. 2001 Feb;69(2):1202-6. doi: 10.1128/IAI.69.2.1202-1206.2001. Infect Immun. 2001. PMID: 11160023 Free PMC article.
-
Immunological responses of neonates and infants to DNA vaccines.Methods Mol Med. 2006;127:239-51. doi: 10.1385/1-59745-168-1:239. Methods Mol Med. 2006. PMID: 16988458 Review.
-
DNA vaccines.Dev Biol Stand. 1998;95:43-53. Dev Biol Stand. 1998. PMID: 9855413 Review.
Cited by
-
An ultra-low-cost electroporator with microneedle electrodes (ePatch) for SARS-CoV-2 vaccination.Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):e2110817118. doi: 10.1073/pnas.2110817118. Proc Natl Acad Sci U S A. 2021. PMID: 34670842 Free PMC article.
-
Electrotransfer parameters as a tool for controlled and targeted gene expression in skin.Mol Ther Nucleic Acids. 2016 Aug 30;5(8):e356. doi: 10.1038/mtna.2016.65. Mol Ther Nucleic Acids. 2016. PMID: 27574782 Free PMC article.
-
Clinical trial in healthy malaria-naïve adults to evaluate the safety, tolerability, immunogenicity and efficacy of MuStDO5, a five-gene, sporozoite/hepatic stage Plasmodium falciparum DNA vaccine combined with escalating dose human GM-CSF DNA.Hum Vaccin Immunother. 2012 Nov 1;8(11):1564-84. doi: 10.4161/hv.22129. Epub 2012 Nov 1. Hum Vaccin Immunother. 2012. PMID: 23151451 Free PMC article. Clinical Trial.
-
DNA immunization.Curr Protoc Microbiol. 2013 Nov 5;31:18.3.1-18.3.24. doi: 10.1002/9780471729259.mc1803s31. Curr Protoc Microbiol. 2013. PMID: 24510291 Free PMC article.
-
The march toward malaria vaccines.Vaccine. 2015 Nov 27;33 Suppl 4(Suppl 4):D13-23. doi: 10.1016/j.vaccine.2015.07.091. Epub 2015 Aug 29. Vaccine. 2015. PMID: 26324116 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
