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. 2007 Oct;45(10):3439-42.
doi: 10.1128/JCM.01411-07. Epub 2007 Aug 1.

Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

E Castellanos  1 , A Aranaz, B Romero, L de Juan, J Alvarez, J Bezos, S Rodríguez, K Stevenson, A Mateos, L Domínguez
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Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

E Castellanos et al. J Clin Microbiol. 2007 Oct.

Abstract

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

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Figures

FIG. 1.
FIG. 1.
REA patterns obtained after Hpy188-III digestion of the PCR product of the gyrB-2 fragment (897 bp). Lanes: 1 and 6, 100-bp-ladder molecular size markers; 2 and 3, digested PCR products from type III strains; 4 and 5, digested PCR products from type I and II strains, respectively.
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