[Interrelation between changes in RNA transcription and glycine-14C incorporation into protein peculiar to hepatoma PC-1]

Abstract

Incorporation of labelled precursor, glycin-1-14C, into the fraction and individual subfractions of blood serum immunoglobulin G (namely into protein positively reacting in the precipitation test) testifies to the fact that at early stages of the tumour development in the rat organism there occurs an intensive synthesis of protein peculiar to hepatoma PC-1. Actinomycin D in a dose blocking the appearance of the peculiar protein in the blood serum of rats with the tumour has a selective effect on the transcription of liver nuclear RNA in rats with hepatoma PC-1. Its inhibitory effect is most pronounced with respect to ribosomal RNA and one of the fractions of DNA-like RNA--RNA-85. It is observed that the fraction of DNA-like RNA--RNA-63, being stable to the effect of actinomycin D at normal state and with liver regeneration is inhibited to some extent by the antibiotic with the presence of the tumour in the rat organism.