Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Aug 6:7:9.
doi: 10.1186/1471-2210-7-9.

Sodium valproate stimulates potassium and chloride urinary excretion in rats: gender differences

Eitaute Jakutiene  1 , Jurgita Grikiniene, Arunas Vaitkevicius, Marina Tschaika, Janina Didziapetriene, Donatas Stakisaitis
Affiliations

Sodium valproate stimulates potassium and chloride urinary excretion in rats: gender differences

Eitaute Jakutiene et al. BMC Pharmacol. .

Abstract

Background: The diuretic effect of valproates and its relation to urinary potassium (K+) and chloride (Cl-) excretion have not yet been investigated, so the aim of this study was to evaluate the influence of a single dose of sodium valproate (NaVPA) on 24-h urinary K+ and Cl- excretion in young adult Wistar rats of both genders. For measurement of K+ in urine, the same animals and samples as in our earlier publication were used (Pharmacology 2005 Nov, 75:111-115). The authors propose a new approach to the pathophysiological mechanisms of NaVPA effect on K+ and Cl- metabolism. Twenty six Wistar rats were examined after a single intragastric administration of 300 mg/kg NaVPA (13 NaVPA-male and 13 NaVPA-female), 28 control intact Wistar rats (14 males and 14 females) were studied as a control group. The 24-h urinary K+, Cl-, creatinine and pH levels were measured.

Results: Total 24-h diuresis and 24-h diuresis per 100 g of body weight were found to be significantly higher in NaVPA-rats of both genders than in rats of the control group (p < 0.05). The data showed NaVPA to enhance 24-h K+ excretion in NaVPA-males and NaVPA-females with significant gender-related differences: 24-h K+ excretion in NaVPA-male rats was significantly higher than in control males (p = 0.003) and NaVPA-female rats (p < 0.001). Regarding the 24-h K+ excretion, NaVPA-female rats did not show a statistically significant difference versus females of the control group (p > 0.05). 24-h urinary K+ excretion per 100 g of body weight in NaVPA-male rats was significantly higher than in control males (p = 0.025). NaVPA enhanced Cl- urinary excretion: 24-h Cl- urinary excretion, 24-h urinary Cl- excretion per 100 g of body weight and the Cl-/creatinine ratio were significantly higher in NaVPA-male and NaVPA-female rats than in gender-matched controls (p < 0.05). 24-h chloriduretic response to NaVPA in male rats was significantly higher than in female rats (p < 0.05).

Conclusion: NaVPA causes kaliuretic and chloriduretic effects with gender-related differences in rats. Further investigations are necessary to elucidate the mechanism of such pharmacological effects of NaVPA.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Correlation between 24-h diuresis and K+ excretion in NaVPA male and female rats. * – Statistically significant correlation.
Figure 2
Figure 2
Correlation between 24-h diuresis and Cl- excretion in NaVPA male and female rats. * – Statistically significant correlation.
Figure 3
Figure 3
Correlation between urine pH and Cl- excretion in NaVPA male and female rats. * – Statistically significant correlation.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Loscher W. Basic pharmacology of valproate: a review after 35 years of clinical use for the treatment of epilepsy. CNS Drugs. 2002;16:669–694. doi: 10.2165/00023210-200216100-00003. - DOI - PubMed
    1. Balding F, Jr, Geller HM. Sodium valproate enhancement of gamma-aminobutyric acid (GABA) inhibition: electrophysiological evidence for anticonvulsant activity. J Pharmacol Exp Ther. 1981;217:445–450. - PubMed
    1. Grikiniene J, Stakisaitis D, Tschaika M. Influence of sodium valproate on sodium and chloride urinary excretion in rats, gender differences. Pharmacology. 2005;75:111–115. doi: 10.1159/000087505. - DOI - PubMed
    1. Bureu M, Laschet J, Minier F, Chauvel P. Intervention of GABAergic neurotransmission in partial epilepsies. Rev Neurol. 1997;153:S46–S54. - PubMed
    1. Fritschy JM, Mohler H. GABAA-receptor heterogeneity in the adult rat brain: differential regional and cellular distribution of seven major subunits. Comp Neurol. 1995;359:154–194. doi: 10.1002/cne.903590111. - DOI - PubMed