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Comment
. 2007 Aug 14;104(33):13215-6.
doi: 10.1073/pnas.0705890104. Epub 2007 Aug 7.

Hepatitis C virus host cell interactions uncovered

Judith M Gottwein  1 , Jens Bukh
Affiliations
Comment

Hepatitis C virus host cell interactions uncovered

Judith M Gottwein et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Impact of host cell factors on production of infectious HCV particles: study design and results reported by Randall et al. (1). (A) Silencing of 62 host cell proteins by experimental RNAi. Small interfering RNAs (siRNAs) were transfected into Huh7.5 cells. Target mRNA is cleaved by cellular RNA-induced silencing complex RISC due to perfect sequence match to bound siRNA (9). Huh7.5 cells were infected with J6/JFH1 HCV viruses at 24, 48, or 72 h after transfection. HCV infectious titers in cell culture supernatants and intracellular HCV RNA levels were determined 48 h after infection. (B) Silencing of seven components of the miRNA biogenesis/RNAi pathway by experimental RNAi and depletion of miR-122. miRNAs such as miR-122 are transcribed as primary miRNAs (pri-miRNAs) and cleaved by Drosha (cofactor DGCR-8) in the nucleus. After nuclear export, the resulting pre-miRNA is processed by Dicer to form an miRNA duplex. Mature single-stranded miRNAs commonly bind their target mRNAs at several sites close to their 3′ end with imperfect match, resulting in assembly of RISCs and translational repression (14). HCV RNA contains two putative miR-122 binding sites in the untranslated regions (15); however, the mode and implications of miR-122 binding are unclear. Components targeted by Randall et al. are indicated by red flashes. Red boxes show the approximate resulting decrease in HCV infectious titers.
See this image and copyright information in PMC

Comment on

  • Cellular cofactors affecting hepatitis C virus infection and replication.
    Randall G, Panis M, Cooper JD, Tellinghuisen TL, Sukhodolets KE, Pfeffer S, Landthaler M, Landgraf P, Kan S, Lindenbach BD, Chien M, Weir DB, Russo JJ, Ju J, Brownstein MJ, Sheridan R, Sander C, Zavolan M, Tuschl T, Rice CM. Randall G, et al. Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12884-9. doi: 10.1073/pnas.0704894104. Epub 2007 Jul 6. Proc Natl Acad Sci U S A. 2007. PMID: 17616579 Free PMC article.

References

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