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. 2007 Sep 5;129(35):10648-9.
doi: 10.1021/ja0735002. Epub 2007 Aug 9.

Crystal structure of a biosynthetic sulfo-hirudin complexed to thrombin

Chang C Liu  1 , Eric Brustad, Wenshe Liu, Peter G Schultz
Affiliations

Crystal structure of a biosynthetic sulfo-hirudin complexed to thrombin

Chang C Liu et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
(A) Structure of sulfotyrosine (Tys) and amino acid residues that directly or indirectly interact with the sulfate group of Tys in the sulfo-hirudin/thrombin complex. The Fo-Fc electron density map covering Tys63I, K81H, Y76H, and a water molecule was contoured at 1σ. Dashed lines shown in orange are hydrogen bonds. (B) Ni2+ binding site of thrombin. The Fo-Fc electron density map covering Ni2+ was contoured at 5σ. Dashed lines are coordination interactions between Ni2+ and surrounding ligands. Backbone carbons in (A) and (B) are presented in the same color as that of the corresponding secondary structure (helices in cyan, strands in purple, and loops in pink). The side chains of amino acid residues are colored in red for oxygen, light gray for carbon, blue for nitrogen, and green for sulfur. Water molecules are represented as balls and colored in red. The superscripts of amino acid labels represent chain identification: H is the heavy chain of thrombin; L is the light chain of thrombin and I is the inhibitor sulfo-hirudin. Thrombin residues are labeled according to the chymotrypsinogen numbering scheme. (See Table 1 for crystal statistics.)
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References

    1. Markwardt F. Semin Thromb Hemost. 2002;28:405–14. - PubMed
    1. Sohn JH, Kang HA, Rao KJ, Kim CH, Choi ES, Chung BH, Rhee SK. Appl Microbiol Biotechnol. 2001;57:606–13. - PubMed
    1. Stone SR, Hofsteenge J. Biochemistry. 1986;25:4622–4628. - PubMed
    1. Liu CC, Schultz PG. Nat Biotechnol. 2006;24:1436–40. - PubMed
    1. Rydel TJ, Ravichandran KG, Tulinsky A, Bode W, Huber R, Roitsch C, Fenton JW., 2nd Science. 1990;249:277–80. - PubMed

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