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. 2007 Sep;4(9):717-9.
doi: 10.1038/nmeth1078. Epub 2007 Aug 12.

Tomographic phase microscopy

Wonshik Choi  1 , Christopher Fang-Yen, Kamran Badizadegan, Seungeun Oh, Niyom Lue, Ramachandra R Dasari, Michael S Feld
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Tomographic phase microscopy

Wonshik Choi et al. Nat Methods. 2007 Sep.

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

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