Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Oct;45(10):3408-10.
doi: 10.1128/JCM.01393-07. Epub 2007 Aug 15.

Validation of nasopharyngeal sampling and culture techniques for detection of Streptococcus pneumoniae in children in Kenya

Osman Abdullahi  1 , Eva Wanjiru, Robert Musyimi, Nina Glass, J Anthony G Scott
Affiliations

Validation of nasopharyngeal sampling and culture techniques for detection of Streptococcus pneumoniae in children in Kenya

Osman Abdullahi et al. J Clin Microbiol. 2007 Oct.

Abstract

We compared nasopharyngeal swabs against nasal wash cultures for detecting colonizing pneumococci and examined the effect of frozen storage in skim milk-tryptone-glucose-glycerin on culture. Among the 55 children with positive nasal wash cultures, swab cultures were positive for 47 (85%). Of the 96 swabs positive on direct plating, 94 (98%) were positive when recultured after freezing.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Matched-pair tables of the three studies. As there was no evidence of laterality (P = 0.18), the left and right swabs were combined to estimate the sensitivity of a single swab against the gold standard of a positive result on either swab. pos, positive result; neg, negative result; Chi sq, chi-square.
See this image and copyright information in PMC

References

    1. Abdullahi, O., et al. The descriptive epidemiology of Streptococcus pneumoniae and Haemophilus influenzae nasopharyngeal carriage in children and adults in Kilifi District, Kenya. Pediatr. Infect. Dis., in press. - PMC - PubMed
    1. Gibson, L. F., and J. T. Khoury. 1986. Storage and survival of bacteria by ultra-freeze. Lett. Appl. Microbiol. 3:127-129.
    1. Hendley, J. O., M. A. Sande, P. M. Stewart, and J. M. Gwaltney, Jr. 1975. Spread of Streptococcus pneumoniae in Families. I. Carriage rates and distribution of types. J. Infect. Dis. 132:55-61. - PubMed
    1. O'Brien, K., H. Nohynek, et al. 2003. The pneumococcal vaccine trials carriage working group. Report from a WHO working group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae. Pediatr. Infect. Dis. J. 22:133-140. - PubMed

Publication types

MeSH terms

LinkOut - more resources