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. 2007 Oct;45(10):3443-5.
doi: 10.1128/JCM.02367-06. Epub 2007 Aug 15.

Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis

Jaya Garg  1 , Ragini Tilak, Sanjay Singh, Anil Kumar Gulati, Atul Garg, Pradyot Prakash, Gopal Nath
Affiliations

Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis

Jaya Garg et al. J Clin Microbiol. 2007 Oct.

Abstract

In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.

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Figures

FIG. 1.
FIG. 1.
Results of nested PCR of clinical specimens from patients with onychomycosis. Lanes 1 and 18, 100-bp DNA ladder (molecular mass markers); lane 2, positive control (288 bp); lane 3, negative control; lanes 5, 10, 13, and 15, nested-PCR-negative specimens; lanes 4, 6, 7, 8, 9, 11, 12, 14, 16, and 17, nested-PCR-positive specimens.
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