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. 2007 Nov;24(11):2138-45.
doi: 10.1007/s11095-007-9415-0. Epub 2007 Aug 15.

Endocytosis and interaction of poly (amidoamine) dendrimers with Caco-2 cells

Kelly M Kitchens  1 , Amy B Foraker, Rohit B Kolhatkar, Peter W Swaan, Hamidreza Ghandehari
Affiliations

Endocytosis and interaction of poly (amidoamine) dendrimers with Caco-2 cells

Kelly M Kitchens et al. Pharm Res. 2007 Nov.

Abstract

Purpose: To investigate the internalization and subcellular trafficking of fluorescently labeled poly (amidoamine) (PAMAM) dendrimers in intestinal cell monolayers.

Materials and methods: PAMAM dendrimers with positive or negative surface charge were conjugated to fluorescein isothiocyanate (FITC) and visualized for colocalization with endocytosis markers using confocal microscopy. Effect of concentration, generation and charge on the morphology of microvilli was observed using transmission electron microscopy.

Results: Both cationic and anionic PAMAM dendrimers internalized within 20 min, and differentially colocalized with endocytosis markers clathrin, EEA-1, and LAMP-1. Transmission electron microscopy analysis showed a concentration-, generation- and surface charge-dependent effect on microvilli morphology.

Conclusion: These studies provide visual evidence that endocytic mechanism(s) contribute to the internalization and subcellular trafficking of PAMAM dendrimers across the intestinal cells, and that appropriate selection of PAMAM dendrimers based on surface charge, concentration and generation number allows the application of these polymers for oral drug delivery.

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References

    1. Bioconjug Chem. 2006 May-Jun;17 (3):728-34 - PubMed
    1. J Cell Biol. 1985 Jul;101(1):85-95 - PubMed
    1. J Pharm Sci. 1990 Jun;79(6):476-82 - PubMed
    1. Pharm Res. 1997 Jun;14(6):763-6 - PubMed
    1. Am J Pathol. 2004 Nov;165(5):1465-77 - PubMed

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