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. 2008 Feb;53(2):429-35.
doi: 10.1007/s10620-007-9881-6. Epub 2007 Aug 17.

Visceral and somatic hypersensitivity in TNBS-induced colitis in rats

QiQi Zhou  1 , Donald D Price, Robert M Caudle, G Nicholas Verne
Affiliations

Visceral and somatic hypersensitivity in TNBS-induced colitis in rats

QiQi Zhou et al. Dig Dis Sci. 2008 Feb.

Abstract

Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male Sprague-Dawley rats (200-250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral, mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2-28 days following TNBS administration (P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated rats demonstrated somatic hypersensitivity at days 14-28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral and somatic hypersensitivity.

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Figures

Figure 1
Figure 1
Bar graph of colon distension pressures in mmHg vs. days following TNBS treatment. Green indicated Saline, blue indicated ethanol treated rats and red indicated TNBS treated rats. Values are expressed as means ± SEM.
Figure 2
Figure 2
Bar graph of mechanical threshold testing on hind-paws in force/g vs. days following TNBS treatment. Green indicated Saline, blue indicated ethanol treated rats and red indicated TNBS treated rats. Values are expressed as means ± SEM.
Figure 3
Figure 3
Bar graph of thermal sensitivity testing on hind-paws shown as latency vs. days following TNBS treatment. Green indicated Saline, blue indicated ethanol treated rats and red indicated TNBS treated rats. Values are expressed as means ± SEM.
Figure 4
Figure 4
Bar graph of tail flick shown as latency vs. days following TNBS treatment. Green indicated Saline, blue indicated ethanol treated rats and red indicated TNBS treated rats. Values are expressed as means ± SEM.
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