Directed growth and differentiation of stem cells towards neural cell fates using soluble and surface-mediated cues

Abstract

Stem and progenitor cells are helping researchers understand the complex process of mammalian development and also show great promise in treating diseases that are unresponsive to standard therapies. The potential for embryonic stem cells to differentiate into any cell in the body is their great benefit but avoiding co-culture with animal cells and efficiently narrowing cell fate to a single cell type remains challenging. Adult progenitor cells have a more restricted cell fate, but have the potential for use in autologous cell therapies and avoid the ethical issues surrounding the derivation of embryonic stem cell lines. While progress is encouraging, there is much work to be done in directing cells to specific lineages before stem and progenitor cells can be commonly used in clinical settings. This review discusses current techniques used for investigation of the growth and differentiation of stem and progenitor cells, with a focus on neural cell fates.