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. 2007 Jul;9(7):546-55.
doi: 10.1593/neo.07289.

Validation of dynamic contrast-enhanced magnetic resonance imaging-derived vascular permeability measurements using quantitative autoradiography in the RG2 rat brain tumor model

Moira C Ferrier  1 , Hemant Sarin, Steve H Fung, Bawarjan Schatlo, Ryszard M Pluta, Sandeep N Gupta, Peter L Choyke, Edward H Oldfield, David Thomasson, John A Butman
Affiliations

Validation of dynamic contrast-enhanced magnetic resonance imaging-derived vascular permeability measurements using quantitative autoradiography in the RG2 rat brain tumor model

Moira C Ferrier et al. Neoplasia. 2007 Jul.

Abstract

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is widely used to evaluate tumor permeability, yet measurements have not been directly validated in brain tumors. Our purpose was to compare estimates of forward leakage K(trans) derived from DCE-MRI to the estimates K obtained using [(14)C]aminoisobutyric acid quantitative autoradiography ([(14)C]AIB QAR), an established method of evaluating blood-tumor barrier permeability. Both DCE-MRI and [(14)C]AIB QAR were performed in five rats 9 to 11 days following tumor implantation. K(trans) in the tumor was estimated from DCE-MRI using the threeparameter general kinetic model and a measured vascular input function. K(i) was estimated from QAR data using regions of interest (ROI) closely corresponding to those used to estimate K(trans). K(trans) and K(i) correlated with each other for two independent sets of central tumor ROI (R = 0.905, P = .035; R = 0.933, P = .021). In an additional six rats, K(trans) was estimated on two occasions to show reproducibility (intraclass coefficient = 0.9993; coefficient of variance = 6.07%). In vivo blood-tumor permeability parameters derived from DCE-MRI are reproducible and correlate with the gold standard for quantifying blood tumor barrier permeability, [(14)C]AIB QAR.

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Figures

Figure 1
Figure 1
(A) [14C]AIB QAR Ki maps. (B) DCE-MRI Ktrans parameter maps for central tumor slices for each animal. DCE-MRI Ktrans parameter maps are displayed within the 25% threshold ROI.
Figure 2
Figure 2
DCE-MRI Ktrans versus [14C]AIB QAR Ki for central tumor slices for the (A) 75%, (B) 50%, and (C) 25% threshold ROI.
Figure 3
Figure 3
Sample images of tumor ROI and corresponding histograms for animal 1. (A) Images of DCE-MRI ROI (white) for the 25%, 50%, and 75% thresholds. (B) Images of corresponding [14C]AIB QAR ROI (white). (C) Histograms of DCE-MRI Ktrans voxelwise data for the corresponding DCE-MRI ROI threshold with polynomial fit trendlines. (D) Histograms of [14C]AIB QAR Ki pixelwise data for the corresponding [14C]AIB QAR ROI threshold.
Figure 4
Figure 4
Test-retest Ktrans estimates from repeat DCE-MRI experiments for all subjects and excluding the highest Ktrans data point (inset).
See this image and copyright information in PMC

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