[The primary research on relevant factors influencing urease activity of Actinomyces naeslundii]

Abstract

Objectives: To investigate the effects of multiple factors that could influence the urease activity of Actinomyces naeslundii.

Methods: The various biochemical methods were used to investigate the changes of urease activity in Actinomyces naeslundii which was under different environmental conditions.

Results: It was observed that under conditions of nitrogen-limited, glucose-excess and sub-acid environment, the activity of the A. naeslundii urease got the different extent increase extend. With cultured under conditions of pH 6.0, limited nitrogen and excess glucose, Actinomyces naeslundii was able to make the urease activity increase up to 149.7 nmol/min x mg cell protein.

Conclusion: The nitrogen, glucose source and environmental pH are such factors that could influence the activity of the A. naeslundii urease; with the dental plaque cariogenicity enhanced, the A. naeslundii urease activity may increase too.