doi: 10.1016/j.jviromet.2007.07.013.
Epub 2007 Aug 23.
A carboxymethyl-cellulose plaque assay for feline calicivirus
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- PMID: 17719097
- DOI: 10.1016/j.jviromet.2007.07.013
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A carboxymethyl-cellulose plaque assay for feline calicivirus
J Virol Methods.
2007 Dec.
Abstract
The standardization of a plaque assay for feline calicivirus in Crandell Reese feline kidney cells using carboxymethyl-cellulose as an overlay medium is described in this report. This methodology gives comparable counts as compared to the standard assay, and prevents monolayer roll over and peel off, as well as easy medium removal. Cell fixation and staining is performed in a considerably reduced period of time, compared to agarose-based methods.
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