Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Oct;73(20):6678-81.
doi: 10.1128/AEM.00986-07. Epub 2007 Aug 24.

Fine-scale genetic diversity among Burkholderia pseudomallei soil isolates in northeast Thailand

Jana M U'ren  1 , Heidie Hornstra, Talima Pearson, James M Schupp, Benjamin Leadem, Shalamar Georgia, Rasana W Sermswan, Paul Keim
Affiliations

Fine-scale genetic diversity among Burkholderia pseudomallei soil isolates in northeast Thailand

Jana M U'ren et al. Appl Environ Microbiol. 2007 Oct.

Abstract

Burkholderia pseudomallei soil isolates from northeast Thailand were genotyped using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) and multilocus sequence typing (MLST). MLVA identified 19 genotypes within three clades, while MLST revealed two genotypes. These close genetic relationships imply a recent colonization followed by localized expansion, similar to what occurs in an outbreak situation.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Unrooted neighbor-joining phylogram of 68 B. pseudomallei isolates. Numbers on the branches indicate the genotype, and A, B and C indicate the three genetic clades.
FIG. 2.
FIG. 2.
Map of regional study area within Khon Kaen Province. The figure illustrates the local watershed, as well as the locations of the 50 sites from which soil samples were collected. Each site is associated with a local village within the province. The center of each circle represents the location of a positive sampling area. Sites that were not positive for B. pseudomallei are shown as a black circle. Pie charts represent the percentages of isolates among three genetic clades (A, B, and C) for the B. pseudomallei DNA samples. The size of the pie chart is indicative of the number of samples from that site, ranging from one to five.
See this image and copyright information in PMC

References

    1. Ashdown, L. R., and S. G. Clarke. 1992. Evaluation of culture techniques for isolation of Pseudomonas pseudomallei from soil. Appl. Environ. Microbiol. 58:4011-4015. - PMC - PubMed
    1. Chaowagul, W., N. J. White, D. A. Dance, Y. Wattanagoon, P. Naigowit, T. M. Davis, S. Looareesuwan, and N. Pitakwatchara. 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J. Infect. Dis. 159:890-899. - PubMed
    1. Cheng, A. C., and B. J. Currie. 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin. Microbiol. Rev. 18:383-416. - PMC - PubMed
    1. Cheng, A. C., D. Godoy, M. Mayo, D. Gal, B. G. Spratt, and B. J. Currie. 2004. Isolates of Burkholderia pseudomallei from northern Australia are distinct by multilocus sequence typing, but strain types do not correlate with clinical presentation. J. Clin. Microbiol. 42:5477-5483. - PMC - PubMed
    1. da Silva Costa, J. L., and V. C. de Oliveira. 2003. Enhancement of soil DNA extraction by the use of hand held mixer. Braz. J. Microbiol. 34:311-312.

Publication types

MeSH terms

LinkOut - more resources