[Effects of Cordyceps sinensis, rhubarb and serum renotropin on tubular epithelial cell growth]

Abstract

Primary cultured rat tubular epithelium was utilized to investigate the effect of Cordyceps sinensis (CS) on cellular proliferation and metabolism. Judging from incorporation rate of 3H-TdR, it was found that the addition of serum containing CS metabolites into the culture media could promote the DNA synthesis of tubular cells profoundly (P less than 0.001). In association with its beneficial effects on gentamycin nephrotoxity in vivo study, it is indicated that CS could enhance the regeneration of injured tubular cells. In addition, sera obtained from 5/6 nephrectomized rats (5/6 NT) and rhubarb treated rats were studied to see their effects on tubular cells growth. The results showed that the serum of 5/6 NT rats could promote the DNA synthesis of tubular epithelium, while the presence of experimental serum of rhubarb in culture median markedly inhibited the DNA synthesis of cells.