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Comparative Study
. 1991 Nov;29(11):2468-72.
doi: 10.1128/jcm.29.11.2468-2472.1991.

Identification of mycobacteria by high-performance liquid chromatography

W R Butler  1 , K C Jost Jr, J O Kilburn
Affiliations
Comparative Study

Identification of mycobacteria by high-performance liquid chromatography

W R Butler et al. J Clin Microbiol. 1991 Nov.

Abstract

Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.

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References

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