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. 1991 Nov;29(11):2517-21.
doi: 10.1128/jcm.29.11.2517-2521.1991.

Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae

H Shirai  1 , M Nishibuchi, T Ramamurthy, S K Bhattacharya, S C Pal, Y Takeda
Affiliations

Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae

H Shirai et al. J Clin Microbiol. 1991 Nov.

Erratum in

  • J Clin Microbiol 1992 Jun;30(6):1620

Abstract

We report a set of oligonucleotide primers and amplification conditions for the polymerase chain reaction to detect the ctx operon of Vibrio cholerae. The results of this assay on strains of V. cholerae and related organisms were identical with those obtained by the DNA colony hybridization test with the polynucleotide probe. The detection limit of this system was 1 pg of chromosomal DNA or broth culture containing three viable cells. The polymerase chain reaction method could directly detect the ctx operon sequences in rice-water stool samples from patients with cholera.

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