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. 1991 Sep;29(9):2047-50.
doi: 10.1128/jcm.29.9.2047-2050.1991.

Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera

M Musiani  1 , M Zerbini, D Gibellini, G Gentilomi, S Venturoli, G Gallinella, E Ferri, S Girotti
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Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera

M Musiani et al. J Clin Microbiol. 1991 Sep.

Abstract

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.

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References

    1. J Med Virol. 1985 Feb;15(2):163-72 - PubMed
    1. Blood. 1986 May;67(5):1411-7 - PubMed
    1. J Biolumin Chemilumin. 1989 Jul;4(1):99-111 - PubMed
    1. J Immunol Methods. 1989 Dec 20;125(1-2):177-83 - PubMed
    1. J Virol Methods. 1989 Jun;24(3):335-46 - PubMed

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