Comparative Study
doi: 10.1128/jcm.29.9.2056-2058.1991.
Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay
Affiliations
- PMID: 1774334
- PMCID: PMC270260
- DOI: 10.1128/jcm.29.9.2056-2058.1991
Item in Clipboard
Comparative Study
Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay
J Clin Microbiol.
1991 Sep.
Abstract
A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved.
Similar articles
-
Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses.Clin Vaccine Immunol. 2006 May;13(5):553-5. doi: 10.1128/CVI.13.5.553-555.2006. Clin Vaccine Immunol. 2006. PMID: 16682475 Free PMC article.
-
Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi.J Clin Microbiol. 1992 Dec;30(12):3122-6. doi: 10.1128/jcm.30.12.3122-3126.1992. J Clin Microbiol. 1992. PMID: 1280648 Free PMC article.
-
Standardisation and comparison of serial dilution and single dilution enzyme linked immunosorbent assay (ELISA) using different antigenic preparations of the Babesia (Theileria) equi parasite.Vet Res. 2003 Jan-Feb;34(1):71-83. doi: 10.1051/vetres:2002055. Vet Res. 2003. PMID: 12588685
-
Recently developed methods for the detection of babesial infections.Trans R Soc Trop Med Hyg. 1989;83 Suppl:21-3. doi: 10.1016/0035-9203(89)90598-1. Trans R Soc Trop Med Hyg. 1989. PMID: 2696156 Review.
-
Conserved recombinant antigens of Anaplasma marginale and Babesia equi for serologic diagnosis.Vet Parasitol. 1995 Mar;57(1-3):93-6. doi: 10.1016/0304-4017(94)03113-b. Vet Parasitol. 1995. PMID: 7597796 Review.
Cited by
-
Theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor.Parasit Vectors. 2015 Jan 20;8:33. doi: 10.1186/s13071-014-0611-6. Parasit Vectors. 2015. PMID: 25600252 Free PMC article.
-
The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species.Infect Immun. 1992 Dec;60(12):5139-44. doi: 10.1128/iai.60.12.5139-5144.1992. Infect Immun. 1992. PMID: 1280624 Free PMC article.
-
Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses.Clin Vaccine Immunol. 2006 May;13(5):553-5. doi: 10.1128/CVI.13.5.553-555.2006. Clin Vaccine Immunol. 2006. PMID: 16682475 Free PMC article.
-
Identification of antigens diagnostic for European isolates of Babesia equi by two-dimensional electrophoresis and western blotting.Parasitol Res. 1994;80(3):182-5. doi: 10.1007/BF00932671. Parasitol Res. 1994. PMID: 8036229
-
Determination of the optimal cutoff value for a serological assay: an example using the Johne's Absorbed EIA.J Clin Microbiol. 1993 May;31(5):1256-61. doi: 10.1128/jcm.31.5.1256-1261.1993. J Clin Microbiol. 1993. PMID: 8501227 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
