doi: 10.1016/s0021-9673(00)86786-2.
Separation of vitamin D metabolites and their analogues by high-pressure liquid chromatography
- PMID: 177435
- DOI: 10.1016/s0021-9673(00)86786-2
Item in Clipboard
Separation of vitamin D metabolites and their analogues by high-pressure liquid chromatography
J Chromatogr.
.
Abstract
The separation of vitamin D3 metabolites and their analogues by high-pressure liquid chromatography was investigated using a Zorbax SIL column. Each metabolite can be separated effectively with a solvent consisting of 2% methanol-methylene chloride or gradient elution from 0.02% to 6% methanol-methylene chloride. The C-24 epimers of 1alpha, 24-dihydroxy- and 1alpha,24,25-trihydroxy-vitamin D3 are resolved as their free forms, while the epimers of 24-hydroxy- and 24,25-dihydroxy-vitamin D3 can be separated as their trimethylsilyl derivatives.
Similar articles
-
Purification of plasma vitamin D metabolites for radioimmunoassay.Clin Chim Acta. 1980 Dec 8;108(2):239-46. doi: 10.1016/0009-8981(80)90010-8. Clin Chim Acta. 1980. PMID: 6969637
-
A radioimmunoassay for 1,25-dihydroxycholecalciferol.Clin Sci Mol Med. 1978 Mar;54(3):329-32. doi: 10.1042/cs0540329. Clin Sci Mol Med. 1978. PMID: 630809
-
Protein binding assays for 25-hydroxy, 24,25-dihydroxy and 1,25-dihydroxy metabolites of vitamin D1) in human plasma.J Clin Chem Clin Biochem. 1981 Oct;19(10):1043-50. doi: 10.1515/cclm.1981.19.10.1043. J Clin Chem Clin Biochem. 1981. PMID: 6975800
-
Studies of the internalization of vitamin D3 metabolites by cultured osteogenic sarcoma cells and their application to a non-chromatographic cytoreceptor assay for 1,25-dihydroxyvitamin D3.Biochem Biophys Res Commun. 1980 Jul 31;95(2):596-602. doi: 10.1016/0006-291x(80)90826-8. Biochem Biophys Res Commun. 1980. PMID: 6251814 No abstract available.
-
Isolation and identification of 23,25-dihydroxyvitamin D3, an in vivo metabolite of vitamin D3.Biochemistry. 1981 Jun 23;20(13):3875-9. doi: 10.1021/bi00516a032. Biochemistry. 1981. PMID: 6268149
Cited by
-
Salmon calcitonin-induced stimulation of 1 alpha,25-dihydroxycholecalciferol synthesis in rats involving a mechanism independent of adenosine 3':5'-cyclic monophosphate.Biochem J. 1979 Nov 15;184(2):269-75. doi: 10.1042/bj1840269. Biochem J. 1979. PMID: 230832 Free PMC article.
-
1 alpha,25-Dihydroxyvitamin D3 and 1 alpha-hydroxyvitamin D3 prolong survival time of mice inoculated with myeloid leukemia cells.Proc Natl Acad Sci U S A. 1983 Jan;80(1):201-4. doi: 10.1073/pnas.80.1.201. Proc Natl Acad Sci U S A. 1983. PMID: 6296868 Free PMC article.
-
Vitamin D metabolism and its possible role in the developing chick embryo.Biochem J. 1981 Jan 15;194(1):103-9. doi: 10.1042/bj1940103. Biochem J. 1981. PMID: 7305973 Free PMC article.