Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae
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- PMID: 1776953
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Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae
Biochem Int.
1991 Jul.
Abstract
Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae has transglycosylation activity. Treatment of (Man)6(GlcNAc)2Asn with the enzyme in the presence of free N-acetylglucosamine gave a mixture of (Man)6GlcNAc and (Man)6GlcNAc beta 1----4GlcNAc mixture. N-Acetylglucosamine at the reducing end of the latter sugar chain was found by HPLC of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 MHz 1H-NMR spectroscopy.
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