doi: 10.1126/science.152.3720.378.
Zonal ultracentrifuge for the separation of ribosomal subunits
- PMID: 17775173
- DOI: 10.1126/science.152.3720.378
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Zonal ultracentrifuge for the separation of ribosomal subunits
Science.
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Abstract
Ribosomal subunits were prepared by means of the Anderson zonal ultracentrifuge. With this technique it is possible to separate more than 300 milligrams of subunits with complete resolution in a single run, as compared with about 2 milligrams with conventional rotors. Superior resolution is achieved in the zonal ultracentrifuge, affording possibilities for the detection and preparation of minor ribosomal components.
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