[A rapid method of preparing the (H3-H4-H2A-H2b)(2) histone octamer in large quantities]
[Article in
Russian]
- PMID: 1777524
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[A rapid method of preparing the (H3-H4-H2A-H2b)(2) histone octamer in large quantities]
[Article in
Russian]
Biokhimiia.
1991 Oct.
Abstract
A simple and fast method for isolation of large amounts of the histone octamer (H2A-H2B-H3-H4)2 is proposed. This method is based on chromatin adsorption by hydroxyapatite with subsequent extraction of the histone octamer with 50 mM sodium-phosphate buffer containing 4 M NaCl pH 8.0. It was shown that the properties of the histone octamer isolated by this extractive procedure are identical with those of the histone octamer obtained by elution on a Sephadex G-100 column. The histone tetramer (H3-H4)2 and dimer (H2A-H2B) were obtained after gel filtration on Sephadex G-100 in 50 mM sodium-acetate (pH 5.6).
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