Effect of anatomical site on insulin action and insulin receptor phosphorylation in isolated rat adipocytes

Abstract

The effects of insulin were evaluated on adipocytes isolated from three different anatomical sites in male, Sprague-Dawley rats: epididymal (EPI), retroperitoneal (RP), and dorsal subcutaneous (SC). The results indicated that maximal insulin-stimulated glucose transport was significantly lower (P less than 0.001) in cells from the SC region as compared to EPI and RP cells. In addition, the ED50 value for SC cells (259 +/- 34 pmol/l) was significantly higher than for EPI (66 +/- 5 pM) or RP adipocytes (111 +/- 32 pmol/l). Insulin inhibition of catecholamine-induced lipolysis was also significantly greater (P less than 0.001) in EPI cells as compared to RP or SC adipocytes, and that was true when expressed in absolute or relative terms. The decrease in the ability of insulin to either stimulate glucose transport or inhibit catecholamine induced lipolysis in SC cells was associated with a decrease in insulin receptor autophosphorylation and receptor tyrosine kinase activity. These data show that insulin action on isolated adipocytes varies as a function of anatomical site, and that these changes are associated with variations in insulin receptor autophosphorylation and insulin receptor tyrosine kinase activity.