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. 1976 Jul 16;193(4249):227-9.
doi: 10.1126/science.193.4249.227.

Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse

A J Campillo, V H Kollman, S L Shapiro

Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse

A J Campillo et al. Science. .

Abstract

New data on intensity-dependent lifetimes indicate that all previous in vivo fluorescence studies of chlorophyll by picosecond techniquies must be reinterpreted. Anomalously short lifetimes result from high-intensity effects due to exciton-excition annihilation processes. Measurements in Chlorella pyrenoidosa with single-pulse, low-intensity excitation indicate a longer "true" lifetime of 650 +/- 150 picoseconds.

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