doi: 10.1126/science.143.3612.1334.
Brucella-Agglutinating Antibodies: Relation of Mercaptoethanol Stability to Complement Fixation
- PMID: 17799243
- DOI: 10.1126/science.143.3612.1334
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Brucella-Agglutinating Antibodies: Relation of Mercaptoethanol Stability to Complement Fixation
Science.
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Abstract
Brucella-agglutinating antibodies from selected bovine blood serums and milk samples did not fix complement, and after treatment with 2-mercaptoethanol they lost agglutinating power. After infection of calves with Brucella abortus, strain 19, agglutinins for Brucella that were inactivated by mercaptoethanol appeared earlier than those stable to mercaptoethanol. Under the conditions of these experiments, the appearance and development of complement-fixing capacity coincided closely with the mercaptoethanol stability of the agglutinins for Brucella.
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