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. 1963 Jun 21;140(3573):1339-40.
doi: 10.1126/science.140.3573.1339.

Microdetermination of Calcium by Aequorin Luminescence

O Shimomura, F H Johnson, Y Saiga

Microdetermination of Calcium by Aequorin Luminescence

O Shimomura et al. Science. .

Abstract

A bioluminescent protein, aequorin, isolated from the jellyfish Aequorea in dilute disodium ethylenediaminetetraacetate solution, emits light on addition specifically of Ca(++) or Sr(++), thus providing the basis for a simple, quantitative micromethod for the determination of these cations, especially in biological fluids.

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