Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007;176(1):70-81.
doi: 10.1111/j.1469-8137.2007.02160.x.

Cotton GhDREB1 increases plant tolerance to low temperature and is negatively regulated by gibberellic acid

Da-Peng Shan, Jin-Guang Huang, Yu-Tao Yang  1 , Ying-Hui Guo  1 , Chang-Ai Wu  1 , Guo-Dong Yang  1 , Zheng Gao  1 , Cheng-Chao Zheng  1
Affiliations
Free article

Cotton GhDREB1 increases plant tolerance to low temperature and is negatively regulated by gibberellic acid

Da-Peng Shan et al. New Phytol. 2007.
Free article

Abstract

The transcription factors C-repeat binding factors/dehydration-responsive element binding proteins (CBFs/DREBs) control the expression of many stress-inducible genes in Arabidopsis. A cDNA clone, designated GhDREB1, was isolated from cotton (Gossypium hirsutum) by cDNA library screening. Northern blot analysis indicated that mRNA accumulation of GhDREB1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (ABA) or drought stress in cotton seedlings. Transgenic tobacco (Nicotiana tabacum) plants overexpressing GhDREB1 displayed stronger chilling tolerance than wild-type plants. Their leaf chlorophyll fluorescence, net photosynthetic rate and proline concentrations were higher than those of control plants during low-temperature treatment. However, under normal growth conditions, the transgenic tobacco plants exhibited retarded growth and delayed flowering. Interestingly, GhDREB1 transcripts in cotton seedlings were negatively regulated by gibberellic acid (GA(3)) treatment. Analysis of the promoter of the GhDREB1 gene revealed the presence of one low-temperature and four gibberellin-responsive elements. Green fluorescent protein (GFP) signal intensity or beta-glucuronidase (GUS) activity driven by the GhDREB1 promoter was clearly enhanced by low temperature but repressed by GA(3). These results suggest that GhDREB1 functions as a transcription factor and plays an important role in improving cold tolerance, and also affects plant growth and development via GA(3).

PubMed Disclaimer

References

    1. Agarwal M, Hao Y, Kapoor A, Dong CH, Fujii H, Zheng X, Zhu JK. 2006. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. Journal of Biological Chemistry 281: 37636-37645.
    1. Ashraf M. 2002. Salt tolerance of cotton: some new advances. Critical Reviews in Plant Sciences 21: 1-32.
    1. Baker SS, Wilhelm KS, Thomashow MF. 1994. The 5′-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Molecular Biology 24: 701-713.
    1. Cheong YH, Kim K-N, Pandey GK, Gupta R, Grant JJ, Luan S. 2003. CBL1, a calcium sensor that differentially regulates salt, drought, and cold responses in Arabidopsis. Plant Cell 15: 1833-1845.
    1. Chinnusamy V, Ohta M, Kanrar S, Lee BH, Hong X, Agarwal M, Zhu JK. 2003. ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes and Development 17: 1043-1054.

Publication types

MeSH terms

LinkOut - more resources