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. 2007 Nov;45(11):3764-7.
doi: 10.1128/JCM.00964-07. Epub 2007 Sep 5.

Genetic diversity of paired middle-ear and pharyngeal nontypeable Haemophilus influenzae isolates from children with acute otitis media

Zachary J Berrens  1 , Carl F Marrs, Melinda M Pettigrew, Sara A Sandstedt, Mayuri Patel, Janet R Gilsdorf
Affiliations

Genetic diversity of paired middle-ear and pharyngeal nontypeable Haemophilus influenzae isolates from children with acute otitis media

Zachary J Berrens et al. J Clin Microbiol. 2007 Nov.

Abstract

Pulsed-field gel electrophoresis was used to determine genetic diversities of multiple nontypeable Haemophilus influenzae isolates from throat and ear specimens of eight children with otitis media. From five children, all ear and throat isolates were identical. The bacterial populations in these specimens showed less diversity than populations in throat isolates of healthy children.

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Figures

FIG. 1.
FIG. 1.
PFGE patterns of isolates from three children, P6 (lanes 1 to 4), K34 (lanes 5 to 8), and P5 (lanes 9 to 12), with OM. Isolates P6ME2.9 (lane 4), K34N2.6 (lane 5), and P5N2.8 (lane 12) each differ from the other isolates collected from P6, K34, and P5, respectively. Lanes M contain the size marker.
FIG. 2.
FIG. 2.
Dendrogram (generated using BioNumerics software with the optimization setting at 1.2% and the position tolerance setting at 1.2%) of isolates digested with SmaI from children with OM. Isolates with a similarity value of greater than 85% (indicated by the arrow at the top) were considered identical.
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