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. 2007 Nov;45(11):3802-3.
doi: 10.1128/JCM.01127-07. Epub 2007 Sep 5.

Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species

Megan E Reller  1 , Amanda B Mallonee, Nicole P Kwiatkowski, William G Merz
Affiliations

Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species

Megan E Reller et al. J Clin Microbiol. 2007 Nov.

Abstract

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

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