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. 1966 Oct 14;154(3746):280-1.
doi: 10.1126/science.154.3746.280.

Protoplasts: preparation from higher plants

A W Ruesink, K V Thimanndagger

Protoplasts: preparation from higher plants

A W Ruesink et al. Science. .

Abstract

Protoplasts can be readily prepared from higher-plant tissue with a concentrated Myrothecium cellulase preparation. An ionic osmoticum produced a higher yield and more viable protoplasts than did the nonionic man nitol osmoticum. Addition of polyuronidases or of ethylenediaminetetra-acetate did not increase the yield. Protoplasts could be prepared from root, leaf, and callus tissue as well as from coleoptiles.

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