Two-Dimensional Gel Electrophoresis

Abstract

Improved resolution of serum protein mixtures is effected by electrophoresis, first in a 5 percent acrylamide gel following which a strip of the resolved pattern is embedded in 8 percent gel and subjected to a second electrophoresis separation at right angles to the first. Lactic dehydrogenase enzymes appear as small rectangular spots lying on an oblique straight line passing through the point of initial application.