[Crystallization of free aspartate aminotransferase from chicken heart cytosol]
[Article in
Russian]
- PMID: 1782267
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[Crystallization of free aspartate aminotransferase from chicken heart cytosol]
[Article in
Russian]
Biokhimiia.
1991 Aug.
Abstract
Homogeneous aspartate aminotransferase (purity--99%, yield--70%) has been prepared from chicken heart cytosol. The purification procedure included fractionation with ammonium sulfate and ethanol and crystallization. Crystals (0.3 x 0.5 x 2 mm) of the free enzyme were prepared from ammonium sulfate solution and studied by X-ray analysis at 2.5 A resolution.
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