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. 1991 Aug;56(8):1488-94.

[Crystallization of free aspartate aminotransferase from chicken heart cytosol]

[Article in Russian]
V M Kochkina
  • PMID: 1782267

[Crystallization of free aspartate aminotransferase from chicken heart cytosol]

[Article in Russian]
V M Kochkina. Biokhimiia. 1991 Aug.

Abstract

Homogeneous aspartate aminotransferase (purity--99%, yield--70%) has been prepared from chicken heart cytosol. The purification procedure included fractionation with ammonium sulfate and ethanol and crystallization. Crystals (0.3 x 0.5 x 2 mm) of the free enzyme were prepared from ammonium sulfate solution and studied by X-ray analysis at 2.5 A resolution.

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