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Clinical Trial
. 2007 Sep;197(3):289.e1-6.
doi: 10.1016/j.ajog.2007.06.027.

Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes

Daniel Montenegro  1 , Roberto Romero, Beth L Pineles, Adi L Tarca, Yeon Mee Kim, Sorin Draghici, Juan Pedro Kusanovic, Jung-Sun Kim, Offer Erez, Shali Mazaki-Tovi, Sonia Hassan, Jimmy Espinoza, Chong Jai Kim
Affiliations
Clinical Trial

Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes

Daniel Montenegro et al. Am J Obstet Gynecol. 2007 Sep.

Abstract

Objective: The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation.

Study design: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10).

Results: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories.

Conclusion: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.

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Figures

Figure 1
Figure 1. Differential expression of microRNAs with advancing gestational age and histologic chorioamnionitis
A, Changes in miR-214 and miR-338 expression as a function of gestational age analyzed within the group of 10 cases with preterm labor without histologic chorioamnionitis. 13 microRNAs displayed decreasing expression with advancing gestation. This figure illustrates 2 examples. Multiple adjustment for the testing of 157 miRNAs was performed (all P < .05). The y-axis represents units of delta Ct (Ct5S – CtmiRNA), with an arbitrary zero point, so that each unit measures a 2-fold change. The x-axis represents gestational age in weeks. B, Differential expression of microRNAs with histologic chorioamnionitis. Box-and-whisker plots of microRNAs differentially expressed between preterm labor cases with and without histologic chorioamnionitis after multiple units of delta Ct (Ct5S – CtmiRNA), with an arbitrary zero point, so that each unit measures a 2-fold change. The fold change is displayed below each microRNA name.
Figure 1
Figure 1. Differential expression of microRNAs with advancing gestational age and histologic chorioamnionitis
A, Changes in miR-214 and miR-338 expression as a function of gestational age analyzed within the group of 10 cases with preterm labor without histologic chorioamnionitis. 13 microRNAs displayed decreasing expression with advancing gestation. This figure illustrates 2 examples. Multiple adjustment for the testing of 157 miRNAs was performed (all P < .05). The y-axis represents units of delta Ct (Ct5S – CtmiRNA), with an arbitrary zero point, so that each unit measures a 2-fold change. The x-axis represents gestational age in weeks. B, Differential expression of microRNAs with histologic chorioamnionitis. Box-and-whisker plots of microRNAs differentially expressed between preterm labor cases with and without histologic chorioamnionitis after multiple units of delta Ct (Ct5S – CtmiRNA), with an arbitrary zero point, so that each unit measures a 2-fold change. The fold change is displayed below each microRNA name.
See this image and copyright information in PMC

References

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